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Hi @yguillen, have the same question about FRP data analysis. Did you manage to have any clue on this ? Thanks |
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Hi all,
We are analyzing single cell data obtained through the new FRP protocol from 10X (https://www.10xgenomics.com/products/fixed-rna-profiling). Unlike conventional 3' method, this is a probe-based method, meaning that only a collection of genes are surveyed. After analysing this data following typical downstream steps (QC, PCA, UMAP integration, etc) we suspect that there is a bias driven by the restricted collection of probes that are surveyed. Should this type of data be analysed with a probe-based-adapted seurat pipeline? Have you ever implemented seurat in this kind of data?
Thanks!
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